Click here for the full list of publications.
Selected publications:
2023
Recent Thymic Emigrants Require RBPJ-Dependent Notch Signaling to Transition into Functionally Mature Naive T Cells.
Teichman S, Wang H, Lee CR, Mohtashami M, Foerster E, Han J, Trotman-Grant AC, Winer S, Tsui H, Philpott DJ, Zúñiga-Pflücker JC. J Immunol. 2023 Jul 1;211(1):81-90. doi: 10.4049/jimmunol.2300140. PMID: 37154711; PMCID: PMC10330261.
Development and function of FOXP3+ regulators of immune responses.
Golzari-Sorkheh M, Zúñiga-Pflücker JC. Clin Exp Immunol. 2023 Jul 5;213(1):13-22. doi: 10.1093/cei/uxad048. PMID: 37085947; PMCID: PMC10324550.
Induction of Human T Cell Development In Vitro with OP9-DL4-7FS Cells Expressing Human Cytokines.
Mohtashami M, Brauer PM, Zúñiga-Pflücker JC. Methods Mol Biol. 2023;2580:249-260. doi: 10.1007/978-1-0716-2740-2_15. PMID: 36374462.
2022
Thymus Reconstitution in Young and Aged Mice Is Facilitated by In Vitro-Generated Progenitor T Cells.
Mohtashami M, Li YR, Lee CR, Zúñiga-Pflücker JC. Front Immunol. 2022 Jul 8;13:926773. doi: 10.3389/fimmu.2022.926773. eCollection 2022. PMID: 35874726; PMCID: PMC9304753.
Realization of the T Lineage Program Involves GATA-3 Induction of Bcl11b and Repression of Cdkn2b Expression.
Thompson PK, Chen ELY, de Pooter RF, Frelin C, Vogel WK, Lee CR, Venables T, Shah DK, Iscove NN, Leid M, Anderson MK, Zúñiga-Pflücker JC. J Immunol. 2022 Jul 1;209(1):77-92. doi: 10.4049/jimmunol.2100366. Epub 2022 Jun 15.PMID: 35705252; PMCID: PMC9248976.
2021
DL4-μbeads induce T cell lineage differentiation from stem cells in a stromal cell-free system.
Trotman-Grant AC, Mohtashami M, De Sousa Casal J, Martinez EC, Lee D, Teichman S, Brauer PM, Han J, Anderson MK, Zúñiga-Pflücker JC. Nat Commun. 2021 Aug 18;12(1):5023. doi: 10.1038/s41467-021-25245-8.PMID: 34408144; PMCID: PMC8373879.
Cutting Edge: TCR-β Selection Is Required at the CD4+CD8+ Stage of Human T Cell Development.
Chen ELY, Brauer PM, Martinez EC, Huang X, Yu N, Anderson MK, Li Y, Zúñiga-Pflücker JC. J Immunol. 2021 May 15;206(10):2271-2276. doi: 10.4049/jimmunol.2100141. Epub 2021 May 3. PMID: 33941655; PMCID: PMC8274368.
High-Oxygen Submersion Fetal Thymus Organ Cultures Enable FOXN1-Dependent and -Independent Support of T Lymphopoiesis.
Han J, Zúñiga-Pflücker JC. Front Immunol. 2021 Mar 30;12:652665. doi: 10.3389/fimmu.2021.652665. eCollection 2021. PMID: 33859647; PMCID: PMC8043069.
2020
Thymic Engraftment by in vitro-Derived Progenitor T Cells in Young and Aged Mice.
Singh J, Mohtashami M, Anderson G, Zúñiga-Pflücker JC. Front Immunol. 2020 Aug 18;11:1850. doi: 10.3389/fimmu.2020.01850. PMID: 32973763; PMCID: PMC7462002.
2019
Generation and function of progenitor T cells from StemRegenin-1-expanded CD34+ human hematopoietic progenitor cells.
Singh J, Chen ELY, Xing Y, Stefanski HE, Blazar BR, Zúñiga-Pflücker JC. Blood Adv. 2019 Oct 22;3(20):2934-2948. doi: 10.1182/bloodadvances.2018026575. PMID: 31648315; PMCID: PMC6849952.
RBPJ-dependent Notch signaling initiates the T cell program in a subset of thymus-seeding progenitors.
Chen ELY, Thompson PK, Zúñiga-Pflücker JC. Nat Immunol. 2019 Nov;20(11):1456-1468. doi: 10.1038/s41590-019-0518-7. Epub 2019 Oct 21. PMID: 31636466; PMCID: PMC6858571.
Close Quarters Can Be a Good Fit for Stem Cells to Become T Cells.
Trotman-Grant A, Zúñiga-Pflücker JC. Cell Stem Cell. 2019 Mar 7;24(3):345-347. doi: 10.1016/j.stem.2019.02.013. PMID: 30849360.
In vitro-generated MART-1-specific CD8 T cells display a broader T-cell receptor repertoire than ex vivo naïve and tumor-infiltrating lymphocytes.
Benveniste PM, Nakatsugawa M, Nguyen L, Ohashi PS, Hirano N, Zúñiga-Pflücker JC. Immunol Cell Biol. 2019 Apr;97(4):427-434. doi: 10.1111/imcb.12231. Epub 2019 Feb 15. PMID: 30633397.
2018
Generation and molecular recognition of melanoma-associated antigen-specific human γδ T cells.
Benveniste PM, Roy S, Nakatsugawa M, Chen ELY, Nguyen L, Millar DG, Ohashi PS, Hirano N, Adams EJ, Zúñiga-Pflücker JC. Sci Immunol. 2018 Dec 14;3(30):eaav4036. doi: 10.1126/sciimmunol.aav4036. PMID: 30552102.
Producing proT cells to promote immunotherapies.
Singh J, Zúñiga-Pflücker JC. Int Immunol. 2018 Nov 14;30(12):541-550. doi: 10.1093/intimm/dxy051. PMID: 30102361; PMCID: PMC6234910.
Integration of T-cell receptor, Notch and cytokine signals programs mouse γδ T-cell effector differentiation.
Zarin P, In TS, Chen EL, Singh J, Wong GW, Mohtashami M, Wiest DL, Anderson MK, Zúñiga-Pflücker JC. Immunol Cell Biol. 2018 Oct;96(9):994-1007. doi: 10.1111/imcb.12164. Epub 2018 Jun 28. PMID: 29754419; PMCID: PMC6197911.
2016
T Cell Genesis: In Vitro Veritas Est?
Brauer PM, Singh J, Xhiku S, Zúñiga-Pflücker JC. Trends Immunol. 2016 Dec;37(12):889-901. doi: 10.1016/j.it.2016.09.008. Epub 2016 Oct 24. PMID: 27789110; PMCID: PMC5135578.
Modeling altered T-cell development with induced pluripotent stem cells from patients with RAG1-dependent immune deficiencies.
Brauer PM, Pessach IM, Clarke E, Rowe JH, Ott de Bruin L, Lee YN, Dominguez- Brauer C, Comeau AM, Awong G, Felgentreff K, Zhang YH, Bredemeyer A, Al-Herz W, Du L, Ververs F, Kennedy M, Giliani S, Keller G, Sleckman BP, Schatz DG, Bushman FD, Notarangelo LD, Zúñiga-Pflücker JC. Blood. 2016 Aug 11;128(6):783-93. doi: 10.1182/blood-2015-10-676304. Epub 2016 Jun 14. PMID: 27301863; PMCID: PMC4982452.
Induction of T Cell Development In Vitro by Delta-Like (Dll)-Expressing Stromal Cells.
Mohtashami M, Zarin P, Zúñiga-Pflücker JC. Methods Mol Biol. 2016;1323:159-67. doi: 10.1007/978-1-4939-2809-5_14. PMID: 26294407.
2015
T cell development runs marrow deep.
Zúñiga-Pflücker JC. J Exp Med. 2015 May 4;212(5):599-600. doi: 10.1084/jem.2125insight3. PMID: 25941319; PMCID: PMC4419345.
Hematopoiesis: from start to immune reconstitution potential.
Liang HC, Zúñiga-Pflücker JC. Stem Cell Res Ther. 2015 Apr 11;6(1):52. doi: 10.1186/s13287-015-0051-z. PMID: 25889009; PMCID: PMC4392461.
Gamma delta T-cell differentiation and effector function programming, TCR signal strength, when and how much?
Zarin P, Chen EL, In TS, Anderson MK, Zúñiga-Pflücker JC. Cell Immunol. 2015 Jul;296(1):70-5. doi: 10.1016/j.cellimm.2015.03.007. Epub 2015 Mar 25. PMID: 25866401.
2014
Dedicated mTEC progenitors stay true, even into adulthood.
Kianizad K, Zúñiga-Pflücker JC. Immunity. 2014 Nov 20;41(5):675-6. doi: 10.1016/j.immuni.2014.11.008. Epub 2014 Nov 20. PMID: 25517607.
New role identified for LRR-containing proteins in T cell development.
Zúñiga-Pflücker JC. J Exp Med. 2014 May 5;211(5):746-7. doi: 10.1084/jem.2115insight4. PMID: 24799532; PMCID: PMC4010890.
An overview of the intrathymic intricacies of T cell development.
Shah DK, Zúñiga-Pflücker JC. J Immunol. 2014 May 1;192(9):4017-23. doi: 10.4049/jimmunol.1302259. PMID: 24748636.
Enforcement of γδ-lineage commitment by the pre-T-cell receptor in precursors with weak γδ-TCR signals.
Zarin P, Wong GW, Mohtashami M, Wiest DL, Zúñiga-Pflücker JC. Proc Natl Acad Sci U S A. 2014 Apr 15;111(15):5658-63. doi: 10.1073/pnas.1312872111. Epub 2014 Mar 31. PMID: 24706811; PMCID: PMC3992653.
Notch signals are required for in vitro but not in vivo maintenance of human hematopoietic stem cells and delay the appearance of multipotent progenitors.
Benveniste P, Serra P, Dervovic D, Herer E, Knowles G, Mohtashami M, Zúñiga- Pflücker JC. Blood. 2014 Feb 20;123(8):1167-77. doi: 10.1182/blood-2013-07-505099. Epub 2013 Dec 20. PMID: 24363404.
2013
Human proT-cells generated in vitro facilitate hematopoietic stem cell-derived T-lymphopoiesis in vivo and restore thymic architecture.
Awong G, Singh J, Mohtashami M, Malm M, La Motte-Mohs RN, Benveniste PM, Serra P, Herer E, van den Brink MR, Zúñiga-Pflücker JC. Blood. 2013 Dec 19;122(26):4210-9. doi: 10.1182/blood-2012-12-472803. Epub 2013 Nov 8. PMID: 24215033; PMCID: PMC5527400.
Induction of T-cell development by Delta-like 4-expressing fibroblasts.
Mohtashami M, Shah DK, Kianizad K, Awong G, Zúñiga-Pflücker JC. Int Immunol. 2013 Oct;25(10):601-11. doi: 10.1093/intimm/dxt027. Epub 2013 Aug 29. PMID: 23988616.
Cellular and molecular requirements for the selection of in vitro-generated CD8 T cells reveal a role for Notch.
Dervovic DD, Liang HC, Cannons JL, Elford AR, Mohtashami M, Ohashi PS, Schwartzberg PL, Zúñiga-Pflücker JC. J Immunol. 2013 Aug 15;191(4):1704-15. doi: 10.4049/jimmunol.1300417. Epub 2013 Jul 12. PMID: 23851691; PMCID: PMC3801448.
Directed differentiation of embryonic stem cells to the T-lymphocyte lineage.
Liang HC, Holmes R, Zúñiga-Pflücker JC. Methods Mol Biol. 2013;1029:119-28. doi: 10.1007/978-1-62703-478-4_9. PMID: 23756946.
Generation, isolation, and engraftment of in vitro-derived human T cell progenitors.
Awong G, Zúñiga-Pflücker JC. Methods Mol Biol. 2013;946:103-13. doi: 10.1007/978-1-62703-128-8_7. PMID: 23179828.
2012
Role of recycling, Mindbomb1 association, and exclusion from lipid rafts of δ-like 4 for effective Notch signaling to drive T cell development.
Shah DK, Mohtashami M, Zúñiga-Pflücker JC. J Immunol. 2012 Dec 15;189(12):5797-808. doi: 10.4049/jimmunol.1202469. Epub 2012 Nov 16. PMID: 23162128.
When three negatives made a positive influence in defining four early steps in T cell development.
Zúñiga-Pflücker JC. J Immunol. 2012 Nov 1;189(9):4201-2. doi: 10.4049/jimmunol.1202553. PMID: 23087424.
Comparative and functional evaluation of in vitro generated to ex vivo CD8 T cells.
Dervovic DD, Ciofani M, Kianizad K, Zúñiga-Pflücker JC. J Immunol. 2012 Oct 1;189(7):3411-20. doi: 10.4049/jimmunol.1200979. Epub 2012 Aug 27. PMID: 22925927.
HES1 opposes a PTEN-dependent check on survival, differentiation, and proliferation of TCRβ- selected mouse thymocytes.
Wong GW, Knowles GC, Mak TW, Ferrando AA, Zúñiga-Pflücker JC. Blood. 2012 Aug 16;120(7):1439-48. doi: 10.1182/blood-2011-12-395319. Epub 2012 May 30. PMID: 22649105; PMCID: PMC3423782.
Notch receptor-ligand interactions during T cell development, a ligand endocytosis-driven mechanism.
Shah DK, Zúñiga-Pflücker JC. Curr Top Microbiol Immunol. 2012;360:19-46. doi: 10.1007/82_2012_225. PMID: 22581027.
2011
On becoming a T cell, a convergence of factors kick it up a Notch along the way.
Thompson PK, Zúñiga-Pflücker JC. Semin Immunol. 2011 Oct;23(5):350-9. doi: 10.1016/j.smim.2011.08.007. Epub 2011 Oct 6. PMID: 21981947.
Thymus-bound: the many features of T cell progenitors.
Awong G, Zuniga-Pflucker JC. Front Biosci (Schol Ed). 2011 Jun 1;3:961-9. doi: 10.2741/200. PMID: 21622245.
Human CD8 T cells generated in vitro from hematopoietic stem cells are functionally mature.
Awong G, Herer E, La Motte-Mohs RN, Zúñiga-Pflücker JC. BMC Immunol. 2011 Mar 23;12:22. doi: 10.1186/1471-2172-12-22. PMID: 21429219; PMCID: PMC3072939.
2010
Determining γδ versus αß T cell development.
Ciofani M, Zúñiga-Pflücker JC. Nat Rev Immunol. 2010 Sep;10(9):657-63. doi: 10.1038/nri2820. Epub 2010 Aug 20. PMID: 20725107.
Key players for T-cell regeneration.
Awong G, LaMotte-Mohs R, Zúñiga-Pflücker JC. Curr Opin Hematol. 2010 Jul;17(4):327-32. doi: 10.1097/MOH.0b013e3283395133. PMID: 20571395.
Direct comparison of Dll1- and Dll4-mediated Notch activation levels shows differential lymphomyeloid lineage commitment outcomes.
Mohtashami M, Shah DK, Nakase H, Kianizad K, Petrie HT, Zúñiga-Pflücker JC. J Immunol. 2010 Jul 15;185(2):867-76. doi: 10.4049/jimmunol.1000782. Epub 2010 Jun 14. Erratum in: J Immunol. 2010 Sep 15;185(6):3777-8. PMID: 20548034.
Positive selection of T cells, an in vitro view.
Dervović D, Zúñiga-Pflücker JC. Semin Immunol. 2010 Oct;22(5):276-86. doi: 10.1016/j.smim.2010.04.014. PMID: 20537907.
γδ and αβ T cell lineage choice: resolution by a stronger sense of being.
Wong GW, Zúñiga-Pflücker JC. Semin Immunol. 2010 Aug;22(4):228-36. doi: 10.1016/j.smim.2010.04.005. Epub 2010 May 13. PMID: 20466561.